MI PathArrays
New MI PathArray™ assays focus on research area-specific pathways in development and disease, bringing the highest microarray sensitivity to your research. Gene expression analysis is often performed using whole-genome microarrays resulting in very large data sets that generate information irrelevant to the focused researcher. Alternative methods for focused gene expression currently utilize qPCR, but these tools have limited gene coverage due to high assay costs and limited instrument capabilities. The MI PathArray microarray platform provides an alternative : a focused gene pathway tool with current scientifically-relevant gene content providing manageable data sets for focused analysis in a cost-effective multi-assay platform.
Focused on Target Content
- Complete probe coverage for the current science
- Continual updates for each probe collection for new discoveries
- New MI PathArray assays introduced monthly
- Four independent assays per slide
| Current MI PathArray assays | Catalog # | # Probes | Genes Covered | |
|---|---|---|---|---|
| Apoptosis | MPA 0300 | 1048 | 994 | more details |
| Growth Factors | MPA0100 | 190 | 142 | more details |
| Inflammation | MPA0200 | 737 | 677 | more details |
| Cancer | MPA0400 | 509 | 465 | more details |
| Transcription Factors | Available Jan 2012 | TBD | TBD | |
| Kinases | Available Feb 2012 | TBD | TBD |
Each multi-assay slide includes four discrete, identical arrays containing pathway-specific collection of 70mer oligonucleotides selected through a vigorous curation process from the MI collection of human gene probes. Probes and controls are printed in triplicate in each assay area offering increased sensitivity and statistical power. Control probes for every assay include 17 probes for widely expressed genes (positive controls), 6 negative probes, and a series of 8 probes for use with spike-in controls used to determine the limits of detectability.
Highly sensitivity and reproducibility
- Highly sensitive results allow semi-quantitative analysis
- Spike-in controls for normalization / semi-quantitation
Designed for use with pre-labeled cDNA derived from total RNA, detailed sample preparation protocols for MI PathArray assays to achieve semi-quantitative results are provided. RNA spike quantities recommended for inclusion included in the reverse transcription and labeling process generate a fluorescence/expression curve to allow semi-quantitative analysis.
Flexible Assays in a Variety of Formats
- Easily used for one- or two- color assays
- Works with your existing sample labeling protocols, if desired
Hybridization your samples on the MI PathArray is readily accomplished using any commercially-available hybridization systems, or with MI-developed protocols for bench-top static or dynamic mixing hybridization methods. Additionally, the printed dimensions of the assay areas have been designed to allow for use in the Tecan HS Pro and other commercially available hybridization systems, as well as with MI's exclusive Dynamic Mixing Hybridization Clamp system compatible with rotisserie hybridization ovens capable of accommodating a 50ml centrifuge tube.
Compatible with Multiple Hybridization Systems
- MI PathArray assays readily used with commercially-available systems
- Easy bench-top processing protocols for non-automated labs
- Dynamic mixing when using rotisserie hybridization ovens
- Hybridization buffer kit available through MI
NEW MI PathArray ASSAYS
- Growth Factors Cat #: MPA0100 .xls
- MI PathArray Growth Factor assays include 190 gene probes covering 142 genes to detect more than 390 transcripts. Each gene is identified to play a role in one or more pathways where human growth factors and growth factor-associated genes are involved in a wide variety of biological processes including differentiation and development. To ensure continuity for researchers using more limited assays for growth factors, MI PathArray assays are fully compatible with other popular assays including PCR arrays and other smaller microarrays.
- Inflammation Cat#: MPA0200.xls
- MI PathArray Inflammation assays include 737 gene probes covering 677 genes to detect more than 2450 transcripts for human genes identified to play a role in pathways associated with inflammation such as chemokines, cytokines, and inflammatory responses including acute-phase and inflammasome complexes. To ensure continuity for researchers using more limited assays for inflammation, MI PathArray assays are fully compatible with other popular assays including PCR arrays and other smaller microarrays.
- Apoptosis Cat#: MPA0300.xls
- MI PathArray Apoptosis assays include 1048 gene probes covering 994 genes which detect more than 3600 transcripts identified to play a role in pathways of apoptosis including activators, inhibitors, and other positive and negative regulators of cell death. To ensure continuity for researchers using more limited assays for apoptosis, MI PathArray assays are fully compatible with other popular assays including PCR arrays and other smaller microarrays.
- cancer Cat#: MPA0400.xls
- MI PathArray Cancer assays include 509 gene probes covering 465 genes which detect more than 1950 transcripts identified to play a role in oncogenesis and the dysregulation of various pathways leading to cancer and metastasis. To ensure continuity for researchers using more limited assays for cancer, MI PathArray assays are fully compatible with other popular assays including PCR arrays and other smaller microarrays.
UPCOMING MI PathArray ASSAYS
MI PathArray TECHNIQUES
- Labeling RNA samples and spike controls .pdf
- Dynamic Mixing Hybridization .pdf
- Static Hybridization .pdf
- Tecan Hybridization Protocol.pdf
- Tecan program for MI Patharray.hpr
Order Form
| MI PathArray ASSAYS | # SLIDES | # ASSAYS (4 per slide) |
Catalog# | PACK LIST PRICE | # PACKS ordered |
promo price per pack | totals | ||
|---|---|---|---|---|---|---|---|---|---|
| Apoptosis w/ static hyb gaskets | 2 | 8 | MPA 0300-02s | $500.00 | x | $300.00 | = | ||
| 5 | 20 | MPA 0300-05s | $1,187.50 | x | $712.50 | = | |||
| 10 | 40 | MPA 0300-10s | $2,125.00 | x | $1,275.00 | = | |||
| Apoptosis w/ dynamic mixing gaskets | 2 | 8 | MPA0100-02d | $540.00 | x | $324.00 | = | ||
| 5 | 20 | MPA0100-05d | $1,282.50 | x | $769.50 | = | |||
| 10 | 40 | MPA0100-10d | $2,295.00 | x | $1,377.00 | = | |||
| Cancer w/ static hyb
gaskets available Dec 2011 |
2 | 8 | MPA0400-02s | $500.00 | x | $300.00 | = | ||
| 5 | 20 | MPA0400-05s | $1,187.50 | x | $712.50 | = | |||
| 10 | 40 | MPA0400-10s | $2,125.00 | x | $1,275.00 | = | |||
| Cancer w/ dynamic mixing
gaskets available Dec 2011 |
2 | 8 | MPA0400-02d | $540.00 | x | $324.00 | = | ||
| 5 | 20 | MPA0400-05d | $1,282.50 | x | $769.50 | = | |||
| 10 | 40 | MPA0400-10d | $2,295.00 | x | $1,377.00 | = | |||
| Inflammation w/ static hyb gaskets | 2 | 8 | MPA0200-02s | $500.00 | x | $300.00 | = | ||
| 5 | 20 | MPA0200-05s | $1,187.50 | x | $712.50 | = | |||
| 10 | 40 | MPA0200-10s | $2,125.00 | x | $1,275.00 | = | |||
| Inflammation w/ dynamic mixing gaskets | 2 | 8 | MPA0200-02d | $540.00 | x | $324.00 | = | ||
| 5 | 20 | MPA0200-05d | $1,282.50 | x | $769.50 | = | |||
| 10 | 40 | MPA0200-10d | $2,295.00 | x | $1,377.00 | = | |||
| Growth Factors w/ static hyb gaskets | 2 | 8 | MPA0100-02s | $500.00 | x | $300.00 | = | ||
| 5 | 20 | MPA0100-05s | $1,187.50 | x | $712.50 | = | |||
| 10 | 40 | MPA0100-10s | $2,125.00 | x | $1,275.00 | = | |||
| Growth Factors w/ dynamic mixing gaskets | 2 | 8 | MPA0100-02d | $540.00 | x | $324.00 | = | ||
| 5 | 20 | MPA0100-05d | $1,282.50 | x | $769.50 | = | |||
| 10 | 40 | MPA0100-10d | $2,295.00 | x | $1,377.00 | = |
| LAB ESSENTIALS - HYBRIDIZATION GASKETS | # SLIDES | # GASKETS (per pack) | Catalog# | LIST PRICE | # PACKS ordered |
PRICE PER PACK | totals | ||
|---|---|---|---|---|---|---|---|---|---|
| Dynamic Mixing gasket, 4-well | 1 | MIDMG001 | $10 | x | $10 | = | |||
| Static HybriWell gasket, 4-well | 1 | MIDMG001 | $5.80 | x | $5.80 | = |
| LAB ESSENTIALS - DYNAMIC MIXING TOOL | ITEMS (per pack) |
Catalog# | LIST PRICE | QNTY | PRICE EACH | totals | |||
|---|---|---|---|---|---|---|---|---|---|
| Dynamic Mixing Starter Kit (2- piece Clamp, Assembly base, Clamp tool) |
1 | MIDMC020 | $112.00 | x | $112.00 | = | |||
| 2-piece Clamp, Dynamic Mixing | 1 | MIDMC001 | $79.00 | x | $79.00 | = | |||
| Assembly base, Dynamic Mixing | MIDMC002 | $55.50 | $55.50 | = | |||||
| Clamp tool, Dynamic Mixing | MIDMC002 | $7.50 | $7.50 | = |
| LAB ESSENTIALS - STATIC HYBRIDIZATION TECHNIQUE | # SLIDES | ITEMS (per pack) |
Catalog# | LIST PRICE | QNTY | PRICE PER PACK | totals | ||
|---|---|---|---|---|---|---|---|---|---|
| Sealing tool for static HybriWell gasket | 1 | MIGST001 | $4.50 | x | $4.50 | = | |||
| Sealing strips for static Hybriwell gasket ports | 5 | MIGSS001 | $5 | x | $5 | = |
| LAB ESSENTIALS - REAGENTS | # SLIDES | ITEMS (per pack) |
Catalog# | LIST PRICE | QNTY | PRICE PER PACK | totals | ||
|---|---|---|---|---|---|---|---|---|---|
| Static Hybridization kit - 60+ rxns (15 slides) | 1 | MIHK001 | $394 | x | $394 | = | |||
| Kit includes: | |||||||||
| • 3mL Long Oligo Hybridization Buffer | |||||||||
| • 500mL Wash A Solution | |||||||||
| • 37.5mL Wash B Solution |
| Dynamic Mixing Hybridization kit - 120+ rxns (40 slides) |
1 | MIHK002 | $450 | x | $450 | = | |||
| Kit includes: | |||||||||
| • 20mL Long Oligo Hybridization Buffer | |||||||||
| • 500mL Wash A Solution | |||||||||
| • 37.5mL Wash B Solution |