MI Technology

The technologies developed at MI span the full spectrum of array production, from early project conception to manufacturing, final QC and product validation. MI's unique ability to meet your every array need is fully supported by our scientists and engineers who develop all of the motion-control software, printing robotics and microfluidic deposition technologies used by MI to provide high throughput capability to any project.

Robotic PLatforms

MI’s unique robots and printing methods readily accommodate any desired array format including:MI proprietary spotting devices support precision and accuracy on the highest density arrays

  • Unusual spatial specifications
  • Replicate numbers
  • Multi-assay requirements
  • Custom-sized printing surfaces

Printing Capillaries

Our innovative microfluidic deposition capillaries feature:

  • Narrow internal diameter to support small molecule arrays
  • Larger internal diameter capillaries accommodate larger molecules and more viscous spotting buffers
  • Proprietary reservoir designs to support any small to large volume print runs

Veriprobe™ Assay

Veriprobe assay developed by MI scientists verifies DNA coupling efficiencies. This proprietary labeling methodology provides:array grid

  • Verification for each probe deposited on the array surface
  • Determines the relative levels of bound probe for each spot
  • Identifies spots with marginal value
  • Discover how MI can accelerate your research efforts by completing the Contact MI form. An MI scientist will contact you at your convenience to discuss your needs.

    Spatial Requirements

    MI scientific and engineering staff will help you define the parameters that best suit your needs. Please complete the Contact MI form; we'll call you to discuss the specific requirements for your arrays.

    MI maintains high levels of spatial precision throughout the printing of every lot to save scanner time and reduce auto-format failures.

    Absolute coordinates of first spot on slide1 X= ± 0.1 mm, Y= ± 0.1 mm
    Space between subarrays Per request, ± 50 um
    Subarray rotation 0 ± 0.2 degrees
    Row spacing within a subarray Per request, ± 20 um
    Spot coordinates within a subarray ± 20 um shift from a perfect grid with (to be specified) pitch in X and Y directions
    Space between array copies Per request, ± 50 um

    1. The absolute placement of the first deposition will be limited by the variability of the slide blank edges, normally ± 100um.
    After placement of the first deposition, tolerances will be as specified in the table to the right. Row and column spacing will be set per request.

    MI Quality

    Our fabrication engineer casts an artistic shadow cast across an array surface while monitoring the manufacturing process.MI offers custom array services and array products providing not only exceptionally precise and accurate spot placement and reproducibility, along with documentation of array quality for each production lot. Validation data for each lot we produce allows you to work in confidence, knowing that MI fully warrants our work to meet your specifications. Every array lot is closely scrutinized before shipping to detect adequate sample deposition, probe coupling, surface chemistry anomalies and DNA or protein probe concentration variations outside of normal limits. This is accomplished through a variety of imaging modalities and assays using randomly harvested slides from each print run. The data is condensed into an easy-to-understand QC report for each order.

    Manufacturing Control

    At MI we work to provide goods and services of exceptional quality. In our efforts to achieve this standard we have implemented:

    • Written procedures for all key processes
    • Monitoring and recording of all processes to ensure their integrity
    • Checking all products for defects, with appropriate and corrective action where necessary
    • Periodic review of all processes and the quality system
    • Continual improvement of all processes

    Deposition Verification

    Verification that important samples are present on your array can impact results. Absent or low signal intensity may not always be an indication of expression level, but rather the result of inadequate or failed sample deposition. MI validates every production lot of arrays using a visual-light scan of representative slides, allowing the identification of missed or inadequately deposited samples. The resultant data denote the exact array and source plate location of any depositions with suspected inadequate material. As a result, MI can validate which samples were deposited and will warranty any array that does not meet your project specifications.

    Coupling efficiencyOur exacting specifications for every aspect of array fabrication [roduces arrays with low variation within and among arrays

    Individual DNA samples may vary in their ability to effectively bind a solid substrate. When these deficiencies go undetected, coupling variability among samples may confound data interpretation. MI's Veriprobe microarray QC assay can improve the overall confidence level of the resultant hybridization data by verifying the relative amounts of probe present and by identifying spots of marginal value.

    To assay coupling efficiency, MI uses our proprietary Veriprobe methodology to attach a fluorescent tag to substrate-bound nucleic acids. This assay is quantitative and provides an accurate means of determining the relative levels of bound probe. An image of the reaction, intensity data, and an overall intensity histogram from a representative slide is provided in the QC report for each lot.

    For protein and other small molecule samples, MI can often incorporate an off-channel fluorophore, which will not interfere with subsequent analysis. This dye can be used to monitor volumetric transfer and spotting morphology. MI can also QC protein arrays using functional assays to validate array quality.

    Discover how MI can accelerate your research efforts by completing the Contact MI form. An MI scientist will contact you at your convenience to discuss your needs.