array

Resources

MI offers highly customized arrays to meet your experimental needs. During the design process several technical terms specific to the physical microarray will be used. We offer our Glossary as a guide to visualizing some of the various options that MI is capable of manufacturing.

Other resources available here include a listing of Publications which relied on microarrays provided by MI. The various publications offer a glimpse into several avenues of investigation where MI microarrays were an integral portion of the study.

Protocols, genelists, gal files and other resources are gathered under the Downloads tab. Many of these same files are also found elsewhere on the MI web site. They are gathered here for your convenience.

MI Glossary

Array – the final product consisting of a series of probes arranged in an organized repeatable format on a substrate suitable for customers experimental conditions.

Assay area - the section of the substrate where the probes are placed.

Deposition - the placement of the probe material onto the substrate surface.

Probe - the material to be deposited onto the microarray surface. Examples are:

  • DNA Oligo
  • DNA (cDNA)
  • Proteins or peptides
  • Antibodies or antigens
  • Cell lysates
  • Small molecules

Gasket - a physical border material applied to the substrate to provide a discrete area for hybridization.

Replicates - often, for statistical and quality assurance purposes, each of the probes are deposited more than once onto the same substrate. Each copy of the probe is termed 'replicate'.

Schematic - a physical illustration of the assay area on the substrate.

Sub replicate – the replicate placement of a probe in close proximity (next to or within the same block) as the initial probe deposition.

Block – the depositions which make up the assay areas on a slide, typically created with an individual pin.

Substrate - the material that will receive the probes. Examples are:

  • Slides
  • Microtiter plates
  • Chips (custom wafer)

Substrate surface - the substrate surface is coated with a selected chemistry to facilitate bonding of the probes to the substrate. The chemistry is selected based on the probe type. Examples are:

  • Amino silane
  • Epoxide
  • Hydrogel
  • Nitrocellulose

Publications

The fungal pathogen Candida albicans autoinduces hyphal morphogenesis by raising extracellular pH

S Vylkova, AJ Carman, HA Danhof, JR Collette, H Zhou, MC Lorenz
mBio 2011 May 17; 2(3):e00055-11.

Transcriptional response of Mycoplasma genitalium to osmotic stress

W Zhang, JB Baseman
Microbiology. 2011 Feb; 157(Pt 2):548-56.

Placental microRNA expression in pregnancies complicated by preeclampsia

DA Enquobahrie, DF Abetew, TK Sorensen, D Willoughby, K Chidambaram, MA Williams
Am J Obstet Gynecol. 2011 Feb; 204(2):178.e12-21

Distinctive DNA methylation patterns of cell-free plasma DNA in women with malignant ovarian tumors

TE Liggett, A Melnikov, Q Yi, C Replogle, W Hu, J Rotmensch, A Kamat, AK Sood, V Levensen
Gynecol Oncol. 2011 Jan; 120(1):113-20.

Responses of human endothelial cells to pathogenic and non-pathogenic Leptospira species

DG Martinez-Lopez, M Fahey, J Coburn
PLoS Negl Trop Dis. 2010 Dec 14; 4(12):e918.

Altered gene expression profiles in the brain, kidney, and lung of deceased neonatal cloned pigs

J Park, SL Marjani, L Lai, M Samuel, D Wax, SR Davis, RS Bruno, RS Prather, X Yang, XC Tian.
Cellular Reprogramming (Formerly "Cloning and Stem Cells"). October 2010, 12(5): 589-597.

PPARγ-induced cardiolipotoxicity in mice is ameliorated by PPARα deficiency despite increases in fatty acid oxidation

Ni-Huiping Son, S Yu, J Tuinei, K Arai, H Hamai, S Homma, GI Shulman, ED Abel, and IJ Goldberg.
J Clin Invest. 2010 October 1; 120(10): 3443–3454.

Microarray analysis of gene expression by skeletal muscle of three mouse models of Kennedy disease/spinal bulbar muscular atrophy

K Mo, Z Razak, P Rao, Z Yu, H Adachi, M Katsuno
PLoS One. 2010 Sep 23;5(9):e12922.

Transcriptome of swarming Proteus mirabilis

MM Pearson, DA Rasko, SN Smith, HL Mobley
Infect Immun. 2010 Jun;78(6):2834-45.

Differential methylation of cell‐free circulating DNA among patients with pancreatic cancer versus chronic pancreatitis

T Liggett, A Melnikov, Q Yi, C Replogle, R Brand… -
Cancer, 2010 April 1, 116(7): 1674–1680.

Methylation patterns of cell-free plasma DNA in relapsing-remitting multiple sclerosis

T Liggett, A Melnikov, S Tilwalli, Q Yi, H Chen, C Replole, X Feng, A Reder, D Stefoski, R Balabanov, V Levenson
J Neurol Sci. 2010 Mar 15; 290(1-2):16-21.

Identification of a modular pathogenicity island that Is widespread among urease-producing uropathogens and shares features with a diverse group of mobile elements

EL Flannery, L Mody, HLT Mobley Infect Immun. 2009 November; 77(11):4887–4894.

Trypanosoma cruzi targets Akt in host cells as an intracellular antiapoptotic strategy.

MV Chuenkova, M PereiraPerrin Sci Signal. 2009 November 17; 2(97): ra74.

Pressure-mediated hypertrophy and mechanical stretch induces IL-1 release and subsequent IGF-1 generation to maintain compensative hypertrophy by affecting Akt and JNK pathways.

S Honsho, S Nishikawa, K Amano, K Zen, Y Adachi, E Kishita, A Matsui, A Katsume, S Yamaguchi, K Nishikawa, K Isoda, DWH Riches, S Matoba, M Okigaki, H Matsubara Circ Res. 2009 Nov 20;105(11):1149-58.

Regulation of expression of the Haemophilus ducreyi LspB and LspA2 proteins by CpxR

M Labandeira-Rey, JR Mock, EJ Hansen
Infect Immun. 2009 Aug; 77(8):3402-11.

Expression profile of mammalian microRNAs in endometrioid adenocarcinoma

W Wu, Z Lin, Z Zhuang, X Liang
European Journal of Cancer Prevention, 2009 February; 18(1):50-55.

Gene and protein expression profiling of the fat-1 mouse brain

D Ménesi, K Kitajka, E Molnár, Z Kis, J Belleger, M Narce, JX Kang, LG Puskas, UN Das
Prostaglandins Leukot Essent Fatty Acids. 2009 Jan; 80(1):33-42.

Gene expression patterns in glucose-stimulated podocytes

SH Han, S Yang, DS Jung, JJ Li, JJ Kim, SJ Kwak, DK Kim, SJ Moon, JE Lee, D-S Han, S-W Kang
Biochemical and Biophysical Research Communications, 2008 June, 6 370(3): 514-518.

Transcriptional interplay among the regulators Rrp2, RpoN and RpoS in Borrelia burgdorferi

Z Ouyang, JS Blevins, MV Norgard
Microbiology. 2008 Sep; 154(Pt 9):2641-58.

Essential role of the response regulator Rrp2 in the infectious cycle of Borrelia burgdorferi

BK Boardman, M He, Z Ouyang, H Xu, X Pang, XF Yang
Infect Immun. 2008 Sep; 76(9):3844-53.

Role of heme in the antifungal activity of the azaoxoaporphine alkaloid sampangine

AK Agarwal, T Xu, MR Jacob, Q Feng, MC Lorenz, LA Walker, AM Clark
Eukaryot Cell. 2008 Feb; 7(2):387-400.

Array-based multiplex analysis of DNA methylation in breast cancer tissues

AA Melnikov, DM Scholtens, EL Wile, SA Khan, VV Levenson
J Mol Diagn. 2008 Jan; 10(1):93-101.

Metabolic analysis of Moraxella catarrhalis and the effect of selected in vitro growth conditions on global gene expression

W Wang, L Reitzer, DA Rasko, MM Pearson, RJ Blick, C Laurence, EJ Hansen
Infect Immun. 2007 Oct;75(10):4959-71

The nonhomologous end joining factor Artemis suppresses multi-tissue tumor formation and prevents loss of heterozygosity

Y Woo, SM Wright, SA Maas, TL Alley, LB Caddle, S Kamdar, J Affourtit, O Foreman1, E C Akeson, D Shaffer, R T Bronson, HC Morse III, D Roopenian and KD Mills
Oncogene. 2007 Sep 6;26(41):6010-20.

Temporal analysis of Candida albicans gene expression during biofilm development

KM Yeater, J Chandra, G Cheng, PK Mukherjee, X Zhao, SL Rodriguez-Zas, KE Kwast, MA Ghannoum, LL Hoyer
Microbiology. 2007 Aug; 153(Pt 8):2373-85.

MicroRNA expression profiling during human cord blood-derived CD34 cell erythropoiesis

ML Choong, HH Yang, I McNiece
Exp Hematol. 2007 Apr; 35(4):551-64.

Lithium treatment prevents neurocognitive deficit resulting from cranial irradiation

EM Yazlovitskaya, E Edwards, D Thotala, A Fu, KL Osusky, WO Whetsell Jr, B Boone, ET Shinohara, DE Hallahan
Cancer Res. 2006 Dec 1; 66(23):11179-86.

Sigma X induces competence gene expression in Streptococcus pyogenes

RL Woodbury, X Wang, CP Moran Jr.
Research in Microbiology, 2006 November; 157(9):851-856.

Metabolic-state-dependent remodeling of the transcriptome in response to anoxia and subsequent reoxygenation in Saccharomyces cerevisiae

LC Lai,1 AL Kosorukoff, PV Burke, KE Kwast
Eukaryot Cell. 2006 Sep; 5(9):1468-89.

Two dual specificity kinases are preferentially induced by wild-type rather than by oncogenic RAS P-21 in Xenopus oocytes

Y Qu, V Adler, T Chu, O Platica, J Michl, S Pestka, L Izotova, M Boutjdir, MR Pincus.
Frontiers in Bioscience. 2006 September 1; 11: 2420-2427.

Identification of Francisella tularensis genes affected by iron limitation

K Deng, RJ Blick, W Liu, EJ Hansen
Infection and Immunity, 2006 July; 74(7): 4224-4236.

RscA, a member of the MDR1 family of transporters, is repressed by CovR and required for growth of Streptococcus pyogenes under heat stress

TL Dalton, JT Collins, TC Barnett, JR Scott
J Bacteriol. 2006 Jan; 188(1):77-85.

Global gene expression analyses of mouse fibroblast L929 cells exposed to IC50 MMA by DNA microarray and confirmation of four detoxification genes' expression by Real-time PCR

A Ishikawa, S Jinno, T Suzuki, T Hayashi, T Kawai, T Mizuno, T Mori, M Hattori
Dental Materials J 2006; 25(2): 205-213.

Unique aspects of gene expression during Candida albicans mating and possible G1 dependency

R Zhao, KJ Daniels, SR Lockhart, KM Yeater, LL Hoyer, DR Soll
Eukaryot Cell. 2005 July; 4(7):1175–1190.

Analysis of the Candida albicans Als2p and Als4p adhesins suggests the potential for compensatory function within the Als family

X. Zhao, S.-H. Oh, K. M. Yeater, and L. L. Hoyer
Microbiology. 2005 May; 151(Pt 5): 1619–1630.

Dynamical remodeling of the transcriptome during short-term anaerobiosis in Saccharomyces cerevisiae: Differential response and role of Msn2 and/or Msn4 and other factors in galactose and glucose media

LC Lai, AL Kosorukoff, PV Burke, KE Kwast
Mol Cell Biol. 2005 May; 25(10): 4075–4091

Chaotic mixer improves microarray hybridization

MK McQuain, K Seale, J Peek, TS Fisher, S Levy, MA Stremler, FR Haselton
Anal Biochem. 2004 Feb 15;325(2):215-26
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Effects of relative humidity and buffer additives on the contact printing of microarrays by quill pins

MK McQuain, K Seale, J Peek, S Levy, FR Haselton
Anal Biochem. 2003 Sep 15;320(2):281-91.

Downloads

Protocols


CURRENT QC FILES

Listed below there are two links for each file type. Each file contains the QC data for the latest production runs. Please carefully match the Lot Number of the file you use with the Lot Number of the arrays you are working with.

catalog # QC Files for MI ReadyArrays
MI ReadyArrays
HS1100 current Human MI ReadyArray
HS1100 past Human MI ReadyArray
MM1100 current Mouse MI ReadyArray
MM1100 past Mouse MI ReadyArray
RN1100 current Rat MI ReadyArray
RN1100 past Rat MI ReadyArray
catalog # QC Files for OpArrays
MI OpArrays
HS1200 current Human OpArray
HS1200 past Human OpArray
MM1200 current Mouse OpArray
MM1200 past Mouse OpArray

GAL FILES

For import into microarray readers and other analysis programs. NOTE: If your computer cannot read this file type, you can add the file extension ".xls" and ready the data using MS Excel.

catalog # GAL Files for MI ReadyArrays
MI ReadyArrays
HS1100 Human MI ReadyArray
MM1100 Mouse MI ReadyArray
RN1100 Rat MI ReadyArray
CF1100 Dog MI ReadyArray
catalog # GAL Files for OpArrays
MI OpArrays
HS1200 Human OpArray
MM1200 Mouse OpArray