Microarrays Inc maintains high levels of spatial precision throughout the printing of every lot. Specifications with regard to absolute array and spot placement are tightly monitored. This attention to detail results in saved scanner time and fewer auto-format failures. The absolute placement of the first deposition will be limited by the variability of the slide blank edges, normally ± 100um. After placement of the first deposition, tolerances will be as specified below. Row and column spacing will be set per request.

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Item	Specification
Absolute coordinates of first spot on slide	X= ± 0.1 mm, Y= ± 0.1 mm
Space between subarrays	Per request, ± 50 um
Subarray rotation	0 ± 0.2 degrees
Row spacing within a subarray	Per request, ± 20 um
Column spacing within a subarray	Per request ± 20 um
Spots coordinates within a subarray	± 20 um shift from a perfect grid with (to be specified) pitch in X and Y directions
Space between array copies	Per request ± 50 um